Thanks every one for the help.
Enhanced gradient version of NOESY-HSQC are working
now.
One of bottle neck in protein solution structure study by
NMR is that we need quite concentrated protein in water.
Now this bottle neck has been enlarged to quite extent due
to the gradient technology. We might be still doing protein
'conditioning' on the protein that I currently study if we did
not have the gradient. Our receiver gain is normally set at
around 512 to 1k for the protein in water solution without
gradient. Now we can set receiver gain to 16K. Using
gradient together with facelift program I now can get pretty
good spectrum of low concentration protein otherwise
beyond my reach.
Regards,
Dr. Sheng Zhong
Dept of Biochem & Biophys
UNC at Chapel Hill
sheng@med.unc.edu