I am helping a friend with some NMR work.
My friend is studying the binding of Cadmium (113Cd) to organic
material in aquatic grasses. The long term application is probably
concerned with either environmental pollution monitoring or clean-up.
He is working from an article which treats the binding of Cd to
Algae. The article is from Environmental Science and Technology
Volume 24 No 9 pp 1309 - 1312.
Investigation of the Metal-Algae Binding Site with 113Cd NMR
Authors: Majidi V; Laude D Jr; and Holcombe J.
So far we are able to obtain the single peak signal from the
113Cd in the CdSO4 solution but we see no shifted peaks indicating
any kind of binding. I am concerned about the sample preparation.
So far as I know we can only obtain signals from nuclei in solution.
It looks as though the freeze dried and finely ground sample is
scarcely soluble in water.
We are placing the sample in a aqueous solution of CDSO4 and adding
200 uL of D2O for locking. The authors of the article ran un- locked.
I wonder why? I am running for typically 12 hours.
I would value any advice on how to prepare the sample and any
thoughts about the work in general.
Sincerely
Andy
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Andy Soper.
School of Pharmaceutical Sciences. Electronics Section.
Rhodes University. P. O. Box 94, 6140 Grahamstown,
South Africa (The 'Rainbow People' Country).
Voice: +27 (0)461 318412 [with answering service]
Cellular Phone +27 (0)82 776 1692
FAX: +27 (0)461 311205
Internet: paas@giraffe.ru.ac.za