Regarding my request for applications of NMR to pharmaceutics and
pharmacology, I have had a couple of interesting messages but the
response has been quite small. Part of the problem is that our 400
MHz magnet probably does not have the resolution required for
working with larger molecules and I guess some drugs are quite
complex.
Another factor is that quite a lot of the research done in Pharmacy
seems to be in the field of Pharmaceutical Chemistry. It is
possible that researchers in other areas of Pharmaceutical Science
may not be fully aware of the possibilities of NMR in their work.
I am waiting for a promised response from Bruker on this subject.
Here follow the responses to date
Sincerely,
Andy
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Dear Andy,
I am running the NMR facilities at the department of pharmacy, ETH
Zuerich/Switzerland.
We have a AMX300 and will get an DRX-500 soon.
Our NMR projects cover the following fields:
1) NMR of peptides and proteins, binding essays by NMR (SAR by NMR)
2) Structure elucidation of natural products
3) small drugs NMR
4) studies of peptides/proteins binding to membranes.
1,2,4 profit from high fields and good equippment of the machines.
We will expand our NMR facilities and havbe a coopoeration with the
chemistry department, with which we ill share another 500 and a 600
MHz machine. Additionally, you need good computing facilities (with
enough disk-space nad printers/plotters, backup media etc..)
hope that helps you a little bit,
Oliver
-- Dr. Oliver Zerbe Dept. Pharmazie ETH-Zuerich Winterthurerstr. 190 CH-8057 Zuerich Tel.: 0041-1-635 60 81/ 635 6080 Fax: 0041-1-635 68 84 mailto:oliver.zerbe@pharma.ethz.ch WWW: http://www.pharma.ethz.ch/~ozXXXXXXXXXXXXXXXXXXXXX ********************************* XXXXXXXXXXXXXXXXXXXXX Andy,
Our machines are used by organic chemists. I think that z gradients with an inverse probe are useful for fast hmqc hmbc and cosy.
hth,
dave scott iowa state university 515-294-4057
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Hi Andy
While I do not work in a pharmacy department, I have been doing some work with pharmaceutical industry and thought I could share a few pointers.
HIGH RES- Here at UNSW we have DMX-600,500 for high res solution on proteins and polysaccharides, but also used heavily by inorganic/organic mufties for the many inverse detected proton experiments WITH gradients coupled to a heteronucleus. eg the typical H-N, H-C, some H-P, but also H-Os, H-Fe, etc.
STRUCTURES - The pharmaceutical guys also like small organics and the 500 is very good with them.
METABOLISM- We also have some biologists studying drug metabolism using whole cells in situ with variable temperature facility. While we have a 10mm probe fr this, they seem happy to use the 5mm probe(an inverse-broad band, tbi 5mm with Z-grad)
MICRO- We are considering buying a micro probe for those really small amounts of natural products that are considered bio-active.
AUTOMATION- We are in the process of installing a DPX-300 with Z-gradient on a QNP probe and a sample changer to do large numbers of samples that might be useful for say quality control. Coupled with the automation NMR(iconnmr), this should allow a greater through-put.
SOLIDS- We also have a solids facility (MSL-300) when some of the pharmaceuticals are powders and sparingly soluble. At present we cannot do high res. 1H/19F detect, but can observe most other nuclei and do routinely C, P , Si some N as well as metals Al, Sn, Hg, Cd.
cheers
jim
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Dr. James Hook Ph 61-2 9385 4689 NMR Facility Fax 61-2-9385 6141 Heffron Building email J.Hook@unsw.edu.au University of NSW http://www.chem.unsw.edu.au/units/nmrfacility.html SYDNEY NSW 2052
"The way is broad, but men love by-ways and side roads"
lao tze
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