Re: 1H/15N hmqc

Rainer Kerssebaum (Rainer.Kerssebaum@bruker.de)
Fri, 6 Nov 1998 07:42:10 -0100

Dear Don,

> I have been attempting to set up a 1H/15N hmqc on a
> DRX-400 running xwinnmr 2.0. I did it by calling in
> the standard Bruker parameters for a 1H/13C hmqc and
> then using edasp to change the nucleus for the second
> dimension to 15N. The experiment worked but I ran
> into problems when I tried to calibrate the chemical
> shift scale for the 15N dimension. The SF value for
> the 15N dimension always sets itself back to 100MHz
> (the 13C frequency) even if I go into edp and explicitly
> set it 40.5MHz(the 15N frequency).
> Does anyone know how to work around this problem?

For all those who didn't set up an 'inverse' experiment
correctly (as indicated in Jane's mail):
Try the AU program "f1ref". This should correct the wrong
referencing. But, you have to reprocess with 'xfb'.

Rainer

-- 
=====================================================================
       ...     ...      Dr. Rainer Kerssebaum
      .    * .    .     Bruker Analytik GmbH
       .   . .   .      Application Lab
       B R U K E R      D-76287 Rheinstetten
       .   . .   .      Voice: +49-721-5161-139    
      .    * .    .     Fax:   +49-721-5161-297
       ...     ...      EMail: Rainer.Kerssebaum@bruker.de
=====================================================================