We've been trying for some months now to obtain satisfactory 2D spectra
from DOSY sequences. on mixtures of mostly small molecules (i.e. not
polymers, biological or otherwise). As far as we can tell, looking at the
individual spectra and the decay curves of their peaks, the data is
probably OK.
The problem comes when we try to process it. Bruker's DOSY processing
program gives results that have very broad, poorly defined lines in the
diffusion dimension, far broader than analysis of the individual peak
decay curves using the T1 package would lead us to expect. The resulting
2D spectra are pretty much useless.
I have recently tried getting the data processed on a friend's Varian
machine, but I ran into another snag. Apparently the conversion of Bruker
data to Varian format gives a result that the Varian machine doesn't
understand.
Can anyone help, either with some advice on the conversion to Varian data
format, or with the actual DOSY processing itself? Does anyone else have
similar problems?
For the technically minded, we are using a DPX400 with XWINNMR 2.1
running on an O2 with Irix 6.3.
Thanks in advance.
Alan
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Dr A S F Boyd Chemistry Department
NMR Spectroscopist Heriot-Watt University
phone: +44-131-451 3214 Riccarton
fax: +44-131-451 3180 Edinburgh
a.s.f.boyd@hw.ac.uk EH14 4AS
http://dava.che.hw.ac.uk/nmr.html Scotland
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