this is a common error from TOO MUCH POWER during acquisition decoupling.
what is your pl16? you should know w/o even looking.
and pcpd3 also (90 degree pulse width for garp or whatever you're using.)
what is your acquistion time? AQ? (that's dw * td). you should know this
too whenever you're doing heteronuclear decoupling, and whereas i generally
use 16K or more points for a 16ppm sw for normal homonuclear 1H expt (such
as watergate), you probably should cut the TD down to 2k-4k range to get
it to an acceptable acquisition time range.
bruker amps have safety protection built into them so as to not fry the
amplifier, and typically there's a duty cycle protection and rf power fault
warning also, but the mismatch condition you're getting happens if your
probe is detuned significantly or if your AQ is too long. i never go over
200ms for 15N decoupling on proteins.
hope this helps,
john chung
the scripps research institute
>===== Original Message From "Chunhua Yuan" <cyuan@ccic.ohio-state.edu> =====
>Dear Bruker Users:
>
>I modified the Bruker p3919gp pulse sequence to select detecting
>15N-attached protons with decoupling during acquisition on a 15N-labeled
>sample. In the try I got an error message in ACB-Display:
> "Status: f3: Amplifier SWR Mismatch. f3: SWR"
>and could not figure it out. The error message would be gone if (1) I
>marked out "cpd3:f3"; or (2) I set DS=0, NS=1 (the error would appear
>after the first scan). The instrument is DMX 600 and software is
>XWINNMR 3.1. Any comments and help will be greatly appreciated.
>
>Thanks,
>
>
>Chunhua Yuan
>Campus Chemical Instrument Center
>The Ohio State University
>
>
>------------------------------------------------------------------------
>------
>;p3919gpf3xf.cy (05/01/20)
>;1D f3-filter wth decoupling during acquisition
>;using f3 - channel
>;water suppression using 3-9-19 pulse sequence with gradients
>;
>#include <Avance.incl>
>#include <Grad.incl>
>#include <Delay.incl>
>
>"d11=30m"
>"d21=1s/(cnst4*2)"
>"DELTA=d19-p21-1.5u"
>"DELTA1=d21-p16-d16-p27*2.385-d19*5"
>"DELTA2=d21-p16-d16-p27*2.154-p0*0.231-d19*5-4u"
>
>1 ze
>2 d1 do:f3
> d11 pl1:f1 pl3:f3
>3 (p1 ph1):f1
> DELTA1 UNBLKGRAD
> p16:gp1
> d16 pl18:f1
> p27*0.231 ph3
> d19*2
> p27*0.692 ph3
> d19*2
> p27*1.462 ph3
> DELTA
> (p21 ph6):f3
> 3u
> (p21 ph8):f3
> DELTA
> p27*1.462 ph4
> d19*2
> p27*0.692 ph4
> d19*2
> p0*0.231 ph4
> 4u
> p16:gp1
> d16
> DELTA2 pl16:f3
> 4u BLKGRAD
> go=2 ph31 cpd3:f3
> d11 do:f3
> wr #0
>exit
>
>ph1=0
>ph3=1
>ph4=3
>ph6=0 0
>ph8=0 2
>ph31=0 2
>
>;pl1 : f1 channel - power level for pulse (default)
>;pl3 : f3 channel - power level for pulse (default)
>;pl16: f3 channel - power level for CPD/BB decoupling
>;pl18: f1 channel - power level for 3-9-19-pulse (watergate)
>;p0 : f1 channel - 90 degree pulse at pl18
>; use for fine adjustment
>;p1 : f1 channel - 90 degree high power pulse
>;p2 : f1 channel - 180 degree high power pulse
>;p16: homospoil/gradient pulse
>;p21: f3 channel - 90 degree high power pulse
>;p22: f3 channel - 180 degree high power pulse
>;p27: f1 channel - 90 degree pulse at pl18
>;d1 : relaxation delay; 1-5 * T1
>;d11: delay for disk I/O [30 msec]
>;d16: delay for homospoil/gradient recovery
>;d19: delay for binomial water suppression
>; d19 = (1/(2*d)), d = distance of next null (in Hz)
>;d21 : 1/(2J)YH
>;cnst4: = J(YH)
>;cpd3: decoupling according to sequence defined by cpdprg3
>;pcpd3: f3 channel - 90 degree pulse for decoupling sequence
>
>;use gradient ratio: gp 1 : gp 2
>; 20 : 20
>;for z-only gradients:
>;gpz1: 20%
>;gpz2: 20%
>;use gradient files:
>;gpnam1: SINE.100
>;gpnam2: SINE.100
>
>
>
>
>--
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