Dear Bruker Users:
Recently I have been performing a number of 1D STD experiments for a
research group here on our AV-500 and the experiments have been working
beautifully. I then thought I would venture onwards and try experiments like
STD-TOCSY/NOESY for epitope mapping purposes. Unfortunately I have hit a
brick wall!
For the record, I am running TopSpin 1.3 pl6 on a Windows 2000 machine. I am
trying to use library pulse sequences such as stdmlevesgpph. After I set up
everything and try to run the sequence I get the warning message: "File Size
#0 resulting from pulse program = 1048576 bytes not consistent with
parameters TD{F1} x TD{F2}x4 = 524288 bytes". The experiment will run but
data appears corrupt. Please note that I can perform an ased with this
sequence without any problems. Also note that the pulse program tells me to
use the parameter set STDMLEVESGPPH but there does not appear to be any such
parameter set, at least with our TopSpin version.
I suspect that the root of this problem is that with every scan the sequence
performs an on/off STD experiment just like in the stddiff.x 1D sequences.
With the 1D sequences a 2 experiment serial file is created for the on and
off experiments - for the 2D's I had thought the subtraction would be done
via the phase cycle but I don't understand the code enough to confirm this
one way or another. If not, do I need a pseudo-3D setup?
I would be most appreciative if someone could advise me as to how I'm
supposed to proceed to get these experiments to first run properly and
secondly how they are supposed to be processed.
Thanks in advance!
Mike
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Michael D. Lumsden, Ph.D.
NMR Facility Coordinator
Room 428, Atlantic Region Magnetic Resonance Centre
Department of Chemistry, Dalhousie University
6274 Coburg Road
Halifax, Nova Scotia, Canada
B3H 4J3
phone: 902-494-1635
FAX: 902-494-1310
http://armrc.chemistry.dal.ca
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