Re: TOCSY

charlie thibault (cth@bruker.com)
Tue, 7 Sep 93 9:36:40 EDT

According to Graham Barlow:
>
> Does anyone know where I can get a TOCSY pulse program for an MSL type
> pulse programmer ie. a .PC file?
>
> Graham
> ._____________________________________________________________________.
> | Dr. Graham Barlow | On the VAX mail: gkb1 |
> | NMR Service | On Tower mail: gkb1 |
> | Dept. of Chemistry | JANET mail: gkb1@UK.AC.YORK |
> | Univ. of York, UK | Internet mail: gkb1@tower.york.ac.uk |
> +----------------------+----------------------------------------------+
>
The following pulse program was submitted by Dr. Stefan Steuernagel
of Bruker Analytische Messtechnik.

-- 
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       ...     ...	Charles G. Thibault
      .    * .    .	Manager of Software Development
       .   . .   .	Bruker Instruments Inc.
       B R U K E R	Billerica, MA. 01821 USA
       .   . .   .	(508)667-9580 x218
      .    * .    .	cth@bii.bruker.com		Internet
       ...     ...     ...!uunet!bii!cth		UUCP


=================================================================
==============================8<------------------------------
; MLEV17PH.PC
; TOCSY/HOHAHA EXPERIMENT WITH MLEV-17 CYCLE FOR MIXING
; SEE A. BAX & D.G. DAVIS, J. MAGN. RESON. 65, 355 (1985)
; PHASE SENSITIVE USING DIGITAL PHASE SHIFTER
; BUFFERED ACQUISITION


DE=DW*3/4               ; CALCULATE DEADTIME DELAY
PROT F1 XT              ; PROTECT F2 AND XT OUTPUTS
VC1=0                   ; PRESET COUNTER FOR EVOLUTION DELAY
VC2=DS+NS               ; PRESET COUNTER FOR AQ WITH DS


START,  10U
        30U     [DECPL=S1]
        LOOP VC2 TIMES
          10U    [PH2 @TPPI RGATE]
          2U 
          D11    [F2 @PLS1 RGATE]         ; PROTON 90 DEG. PULSE
          2U     [PH2 0 RGATE]
          2U
          LOOP VC1 TIMES
            DW   [RGATE]                  ; EVOLUTION
          END LOOP
          30U    [DECPL=S2 RGATE]
        2U
INCLUDE MLEV17F2.INCL                     ; SET MLEV17 PARMS.      
          D3     [STA RGATE]
          D0                              ; ACQUISITION
          10U
        END LOOP
        10U
        2U      [++VC1]                   ; INCREMENT COUNTER FOR
                                          ; EVOLUTION DELAY
        2U      [++TPPI]                  ; INCREMENT LIST FOR
                                          ; DIGITAL PHASE SETTING
        2U      [VC2=NS]                  ; RESET COUNTER FOR AQ
                                          ; WITHOUT DUMMY SCANS
        10U
GOTO START


BEGIN LISTS
TPPI,   0 90 180 270
PLS1,   +X -X -X +X +Y -Y -Y +Y
PLS2,   +X +X -X -X +Y +Y -Y -Y
PLS3,   +Y +Y -Y -Y -X -X +X +X
PLS4,   -X -X +X +X -Y -Y +Y +Y
PLS5,   -Y -Y +Y +Y +X +X -X -X
RLS,    +X -X -X +X +Y -Y -Y +Y
END LISTS


; FOR MLEV-17 PARAMETERS SEE ALSO MLEV17F1.INCL
; SET DP TO GET 90 DEG. PULSE OF CA. 25 US TO YIELD SPIN LOCK FIELD
; OF ABOUT 10 KHZ
; USE O2 FOR CARRIER FREQUENCY, REC=F2
; DECOUPLER MODE: DO
; D11 = 90 DEGREE PULSE WIDTH
; D12 = 180 DEGREE PULSE WIDTH
; D21 = LOW LEVEL 90 DEGREE PULSE
; D22 = LOW LEVEL 180 DEGREE PULSE
; D13 = 2.5 MS, TRIMPULSE
;
;       MIXING-TIME
; C1 = ------------- ,
;         66 * D21
;
; FOR DIRECT CONNECTIVITIES USE CA. 40 MS MIXING TIME
; FOR REMOTE CONNECTIVITIES USE CA. 80 MS MIXING TIME
; NS=8*N
; DS=8
;
; FOR PROCESSING USE:
; MC2=W, ND4=2, REV=N, WDW1=WDW2=Q, SSB1=SSB2=2 OR 3


hear starts the INCLUDE-file:
; MLEV17 FOR TOCSY/HOHAHA EXPERIMENTS INCLUDING TRIM PULSES
; FOR INSTRUMENT SETUP IN INVERSE MODE
; INCLUDE SEQUENCE FOR:
;  MLEV17F2.PC
;  INVMLEVF.PC


D13     [F2 @PLS3 RGATE]
LOOP C1 TIMES           ;SET C1 FOR MIXING TIME
                        ;CYCLE IS 66*D21
D21     [F2 @PLS2 RGATE]
D22     [F2 @PLS3 RGATE]
D21     [F2 @PLS2 RGATE]
D21     [F2 @PLS4 RGATE]
D22     [F2 @PLS5 RGATE]
D21     [F2 @PLS4 RGATE]
D21     [F2 @PLS4 RGATE]
D22     [F2 @PLS5 RGATE]
D21     [F2 @PLS4 RGATE]
D21     [F2 @PLS2 RGATE]
D22     [F2 @PLS3 RGATE]
D21     [F2 @PLS2 RGATE]
D21     [F2 @PLS4 RGATE]
D22     [F2 @PLS5 RGATE]
D21     [F2 @PLS4 RGATE]
D21     [F2 @PLS4 RGATE]
D22     [F2 @PLS5 RGATE]
D21     [F2 @PLS4 RGATE]
D21     [F2 @PLS2 RGATE]
D22     [F2 @PLS3 RGATE]
D21     [F2 @PLS2 RGATE]
D21     [F2 @PLS2 RGATE]
D22     [F2 @PLS3 RGATE]
D21     [F2 @PLS2 RGATE]
D21     [F2 @PLS4 RGATE]
D22     [F2 @PLS5 RGATE]
D21     [F2 @PLS4 RGATE]
D21     [F2 @PLS2 RGATE]
D22     [F2 @PLS3 RGATE]
D21     [F2 @PLS2 RGATE]
D21     [F2 @PLS2 RGATE]
D22     [F2 @PLS3 RGATE]
D21     [F2 @PLS2 RGATE]
D21     [F2 @PLS4 RGATE]
D22     [F2 @PLS5 RGATE]
D21     [F2 @PLS4 RGATE]
D21     [F2 @PLS2 RGATE]
D22     [F2 @PLS3 RGATE]
D21     [F2 @PLS2 RGATE]
D21     [F2 @PLS2 RGATE]
D22     [F2 @PLS3 RGATE]
D21     [F2 @PLS2 RGATE]
D21     [F2 @PLS4 RGATE]
D22     [F2 @PLS5 RGATE]
D21     [F2 @PLS4 RGATE]
D21     [F2 @PLS4 RGATE]
D22     [F2 @PLS5 RGATE]
D21     [F2 @PLS4 RGATE]
D22     [F2 @PLS3 RGATE]      ; PURGE 180 DEGREE PULSE
END LOOP
D13     [F2 @PLS3 RGATE]      ; TRIM PULSE


==============================8<------------------------------

-- 
!!! please notice the new ZIP-code !!!
=====================================================================
       ...     ...      Dr. Stefan Steuernagel
      .    * .    .     Bruker Analytische Messtechnik GmbH
       .   . .   .      Silberstreifen
       B R U K E R      D-76287 Rheinstetten
       .   . .   .      Voice: +49-721-5161-124
      .    * .    .     Fax:   +49-721-517101
       ...     ...      EMail: ste%bamdemo%bamsoft@Germany.EU.net
=====================================================================