RE: working with frequency lists

From: Kerssebaum Rainer <Rainer.Kerssebaum@bruker-biospin.de>
Date: Sat Mar 21 2009 - 21:11:10 EDT

Dear Rudi,

Here is an answer for Markus Hoffmann.

A frequency list always contains the base frequency in the beginning and a 
list of actual offset values, line by line. Example:
O 300.13
1500.0
4500.0

The 'troesyph' PP needs 2 different entries, not 3. First is on-resonance 
for AQ and 2nd is off-resonance for spinlock.


Best Regards

Rainer


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Dr. Rainer Kerssebaum                                   Dr. Rainer 
Kerssebaum
Applikations-Chemiker NMR                 Application Scientist, NMR 
Division
Bruker BioSpin GmbH                                       Bruker BioSpin 
GmbH
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Silberstreifen
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Rheinstetten
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Germany
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                     Rainer.Kerssebaum@bruker-biospin.de
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> Does anyone have experience how to work with pulse programs 
> that use a 
> frequency list?
> We are trying unsuccessfully to use the pulse program 
> "troesyph", which uses 
> a frequency list and comes with Topspin 1.3 (patch level 8 on 
> a windows 
> platform).
>  
> 1.) We created a frequency list "f1" with values 1500, 4500 
> and 1500 as 
> entries. This list was then chosen under FQ1LIST. 
> However, we either get compilation errors or messages of too 
> large values in 
> the frequency list when attempting a RGA. 
> What are we doing wrong with respect to procedure for setting up the 
> frequency list for this pulse program? 
> 2.) After reading the acquisition manual, it is ambiguous to 
> us how the 
> values in the frequency list are treated. Are these absolute 
> values that 
> will be used as entered or will those be added to the O1[Hz] 
> (or SPOFF10
> [Hz]) values? So even after getting the program to work we 
> would still be 
> uncertain what actual off-set values we would obtain. In this 
> regard, is 
> there generally a way to check that the offset for the 
> spin-lock pulse was 
> set properly?
> 3.) Another curious thing is an observation about the looping of this 
> particular pulse program. It seems that the first time 
> through, 3 values 
> are read from the frequency list, thus 3 values should be 
> listed in the 
> frequency list. However, in every following iteration, the 
> loop seems to go 
> back in between the first and second time accessing the 
> frequency list, 
> thus only reading twice from the frequency list. So how many 
> values should 
> be listed in the frequency list?
> 
> 
> Any helpful comments would be much appreciated, 
> Kind Regards, 
> 
> Markus 
> 
> 
> 
> 
> 
> Markus Hoffmann
> Department of Chemistry
> The College at Brockport 
> State University of New York
> Phone: (585) 395-5587
> FAX: (585) 395-5805
> mhoffman@brockport.edu
> 
> 
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Received on Sat Mar 21 18:11:12 2009