Thank you very much for the very helpful hints to solve this basic problem. By working carefully on baseline correction, it made really a big difference and now I can get the absolute integrals reliably. Thank you very much Very happy new year to all Syed On Dec 29, 2010, at 1:04 PM, Chris Canlas wrote: > Yed, > > I am assuming you are trying to measure absolute integration, and > without a reference standard? Please check if they were acquired with > the same receiver gain and number of scans. And then make sure that > you applied the same baseline correction for both. > If you have a reference peak, it might be more straigthforward. > Chris > > > On Wed, Dec 29, 2010 at 6:44 AM, Bruker Users Mail > <bruker-users-mail@chemistry.mcmaster.ca> wrote: >> Please see below a question regarding Saturation Transfer Difference >> experiments. >> >> __________________________________________________________________________ >> >> To subscribe/unsubscribe: Please send a note to >> >> bruker-users-mail-request@chemistry.mcmaster.ca >> >> For subscriptions, please include a list of Bruker instruments you are >> using, see http://www.chemistry.mcmaster.ca/bum/BUM.txt >> E-mail archives are at: >> http://www.chemistry.mcmaster.ca/bum >> ___________________________________________________________________________ >> >> >> ---------- Forwarded message ---------- >> Date: Wed, 29 Dec 2010 09:39:37 -0500 >> From: "Hussan, Syed (NIH/VRC) [E]" <hussans@niaid.nih.gov> >> To: "'bruker-users-mail@chemistry.mcmaster.ca'" >> <bruker-users-mail@chemistry.mcmaster.ca> >> Subject: integration of 1D STD, relaible? >> >> >> Hi Bruker users >> >> I am working with STD experiments to measure the binding constant of a >> ligand. When I use higher ligand excess, I obviously get larger STD effect >> as compared to one which obtained with very little ligand excess. >> Qualitatively it looks fine. But when I integrate an isolated STD peak by >> using Topspin 1.3, I do not get the integral value in accordance with the >> apparent relative signal intensity in two experiments. Ironically, once a >> signal which looks less than 50 % of the other, its integral value is higher >> than the apparent larger signal. Could any one guide me to obtain the >> integral values of STD 1D in a very reliable way. Is there any other >> reliable software other than topspin? I do not have access to xwin any more. >> >> >> With best wishes and happy new year >> >> yed >>Received on Wed Dec 29 13:22:12 2010
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