Re: integration of 1D STD, relaible? (fwd)

From: Hariom Gupta <hhariom.82@gmail.com>
Date: Wed Dec 29 2010 - 10:15:45 EST

Hi syed,

I would like to tell you that the integration of any peaks represent the
area under that correspond peak. it is not a measurement of integration, so
want say you if you getting more integral value that means contrubution for
number of proton will be more for that peak. in you case a peak which have
more integration value but looks small be more broader as compare to your
standard peak.

What happened in your earlier problem, did you got solution of that.


hariom gupta
scientist NMR
Division
CSMCRI, Bhavnagar

On Wed, Dec 29, 2010 at 8:14 PM, Bruker Users Mail <
bruker-users-mail@chemistry.mcmaster.ca> wrote:

> Please see below a question regarding Saturation Transfer Difference
> experiments.
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> ---------- Forwarded message ----------
> Date: Wed, 29 Dec 2010 09:39:37 -0500
> From: "Hussan, Syed (NIH/VRC) [E]" <hussans@niaid.nih.gov>
> To: "'bruker-users-mail@chemistry.mcmaster.ca'"
>    <bruker-users-mail@chemistry.mcmaster.ca>
> Subject: integration of 1D STD, relaible?
>
>
> Hi Bruker users
>
> I am working with STD experiments to measure the binding constant of a
> ligand. When I use higher ligand excess, I obviously get larger STD effect
> as compared to one which obtained with very little ligand excess.
> Qualitatively it looks fine. But when I integrate an isolated STD peak by
> using Topspin 1.3, I do not get the integral value in accordance with the
> apparent relative signal intensity in two experiments. Ironically, once a
> signal which looks less than 50 % of the other, its integral value is
> higher
> than the apparent larger signal. Could any one guide me to obtain the
> integral values of STD 1D in a very reliable way. Is there any other
> reliable software other than topspin? I do not have access to xwin any
> more.
>
>
> With best wishes and happy new year
>
> yed
>

Received on Wed Dec 29 10:15:47 2010