Date/Time
Date(s) - 30/11/2023
1:30 pm - 2:20 pm

Title: Synthesis of DimLe^A, Le^ALe^B and DimLe^X Tumor associated Carbohydrate Antigens Fragments

Date: Thursday November 30

Time: 1:30pm

Room: ABB 165

Host: Dr. Katherine Bujold

Abstract: Despite the interesting potential of the tumor associated carbohydrate antigens (TACAs) dimLe^A and Le^BLe^A to develop anti-cancer immunotherapies, little research has been conducted on these antigens. In our quest to discover fragments of these TACAs that could be targeted for the development of anti-cancer therapeutics, we will describe the synthesis of tri- to pentasaccharide fragments and the challenges encountered. The desired final compounds were eventually obtained following a stepwise approach as nonyl or 9-aminononyl glycosides after one step deprotection reactions in dissolving metal conditions. We will also consider the synthesis of dimeric Lewis X (dimLe^X) TACA fragments lacking the non-reducing end fucosyl residues. The 6-aminohexyl glycoside pentasaccharide was conjugated to BSA and we will present results obtained from titration experiments with  monoclonal antibodies (mAbs) SH2 and IG5F6, which are known to bind polymeric Le^X structures preferentially over the monomeric Le^X antigen.